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KMID : 0379120200480030285
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Korean Journal of Mycology
2020 Volume.48 No. 3 p.285 ~ p.296
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Microbiological Characteristics of Whitening Tyrosinase Inhibitor-producing Wild Yeasts, Saccharomyces cerevisiae WJSL0191 and Papiliotrema laurentii ON30 and Production
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Kim Ji-Yoon
Lee Jong-Soo
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Abstract
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The goal of this study and potent whitening tyrosinase inhibitor-producing wild yeasts and further optimiz production of tyrosinase. Among non-pathogenic wild yeasts obtained from soils in Daejeon city and spice field of Geumsan in Chungcheongnam-do, Korea, we selected Saccharomyces cerevisiae(S. cerevisiae) WJSL0191 and Papiliotrema laurentii (P. laurentii) ON30 show 33.2% and 27.3% respectively. These selected strains formed and not pseudomycelium. S. cerevisiae WJSL0191 was sugar-tolerant as well as halophilic in 20% glucose-containing yeast (YPD) medium and 15% NaCl-containing YPD medium. S. cerevisiae WJSL0191 and P. laurentii ON30 showed 26.2% and 18.6% anti-wrinkle elastase inhibitory activities, respectively. aximal production of tyrosinase inhibitors obtained when S. cerevisiae WJSL0191 was cultured at 30 for 72h in YPD medium and P. laurentii ON30 was incubated at 20℃ for 24hr.
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KEYWORD
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Papiliotrema laurentii ON30, Saccharomyces cerevisiae WJSL0191, Tyrosinase inhibitor, Whitening, Wild yeast
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